Sonodynamic cytotoxic effect of hematoporphyrin monomethyl ether on C6 glioma cells - <i>in vitro</i> study

Wu Yue; Da-yong Song; Zhao Zhao; Shao-dong Cao; Xiang-xi Meng; Xu Zhang; Zheng Huang

doi:10.1117/12.703200

13 February 2007 Sonodynamic cytotoxic effect of hematoporphyrin monomethyl ether on C6 glioma cells: in vitro study

Wu Yue, Da-yong Song, Zhao Zhao, Shao-dong Cao, Xiang-xi Meng, Xu Zhang, Zheng Huang

Proceedings Volume 6438, Biophotonics and Immune Responses II; 64380I (2007) https://doi.org/10.1117/12.703200
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

Objectives: To study the cytotoxicity induced by photosensitizer mediated sonodynamic effects on c6 glioma cells in vitro and to lay the experimental foundation for the sonodynamic therapy (SDT) of glioma tumor. Methods: A domestic photosensitizer hematoporphyrin monomethyl ether (HMME) was used as the sensitizer. The growth inhibition rate of c6 glioma cells at different times after SDT was investigated by MTT assay. The apoptotic rate and cell circle profiles of c6 glioma cells was examined by a flow cytometry. Results: The growth inhibition rate of c6 glioma cells after SDT significantly increased. Ultrasound only group also showed some effects. Whereas, the inhibition rate of HMME only group has no significant difference compared with the control group (without ultrasound and HMME). HMME-mediated SDT increased the apoptotic rate and the percentage of S phase cells but decreased the percentages of G0/G1 and G2/M. SDT also increased the apoptosis and proliferation rate (APR). Conclusions: Our preliminary data indicated that SDT could kill c6 glioma cells in vitro and possibility through induction of apoptosis and arrest cell circle at S/G₂.

Citation Download Citation

Wu Yue, Da-yong Song, Zhao Zhao, Shao-dong Cao, Xiang-xi Meng, Xu Zhang, and Zheng Huang "Sonodynamic cytotoxic effect of hematoporphyrin monomethyl ether on C6 glioma cells: in vitro study", Proc. SPIE 6438, Biophotonics and Immune Responses II, 64380I (13 February 2007); https://doi.org/10.1117/12.703200

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