Calibration of a phase-shifting DIC microscope for quantitative phase imaging

Sharon V. King; Ariel R. Libertun; Chrysanthe Preza; Carol J. Cogswell

doi:10.1117/12.725061

14 February 2007 Calibration of a phase-shifting DIC microscope for quantitative phase imaging

Sharon V. King, Ariel R. Libertun, Chrysanthe Preza, Carol J. Cogswell

Proceedings Volume 6443, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIV; 64430M (2007) https://doi.org/10.1117/12.725061
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

Phase-shifting differential interference contrast (DIC) provides images in which the intensity of DIC is transformed into values linearly proportional to differential phase delay. Linear regression analysis of the Fourier space, spiral phase, integration technique shows these values can be integrated and calibrated to provide accurate phase measurements of objects embedded in optically transparent media regardless of symmetry or absorption properties. This approach has the potential to overcome the limitations of profilometery, which cannot access embedded objects, and extend the capabilities of the traditional DIC microscope, which images embedded phase objects, but does not provide quantitative information.

Citation Download Citation

Sharon V. King, Ariel R. Libertun, Chrysanthe Preza, and Carol J. Cogswell "Calibration of a phase-shifting DIC microscope for quantitative phase imaging", Proc. SPIE 6443, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIV, 64430M (14 February 2007); https://doi.org/10.1117/12.725061

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