Nanometer tracking of single quantum dot array fluorescence: application to bone biomechanics

Kurtulus Golcuk; Thomas M. Vanasse; Michael D. Morris; Steven A. Goldstein

doi:10.1117/12.705551

14 February 2007 Nanometer tracking of single quantum dot array fluorescence: application to bone biomechanics

Kurtulus Golcuk, Thomas M. Vanasse, Michael D. Morris, Steven A. Goldstein

Proceedings Volume 6448, Colloidal Quantum Dots for Biomedical Applications II; 644815 (2007) https://doi.org/10.1117/12.705551
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

We report the use of spatial arrays of single quantum dots (QD) as fluorescent probes to quantify deformations and displacements of bone tissue components (e.g. collagen and carbonated apatite) at the nanometer to micrometer level under mechanical load. Quantum dot bright emission and robustness allow nanometer localization and motion tracking by center of gravity (COG) analysis. Coupons of milled cortical bone are loaded in a purpose-built dynamic mechanical loading system that fits on a microscope stage. We used QD streptavidin conjugates to label the bone specimen prior to mechanical loading. COG of the laser-induced QD fluorescence diffraction spot is measured and tracked in real time (<0.1 sec) as the tissue is loaded quasi-statically. The technique has been validated by comparing the average values of tangent elastic moduli obtained by the QD/COG method to measurements made with an attached micro-strain gage and a calibrated load cell. Two or more colors of QD can be used to measure relative motions of different bone tissue components, as well as to measure small out-of-plane motions that cannot be detected otherwise.

Citation Download Citation

Kurtulus Golcuk, Thomas M. Vanasse, Michael D. Morris, and Steven A. Goldstein "Nanometer tracking of single quantum dot array fluorescence: application to bone biomechanics", Proc. SPIE 6448, Colloidal Quantum Dots for Biomedical Applications II, 644815 (14 February 2007); https://doi.org/10.1117/12.705551

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