Heterodyne interference microscopy for non-invasive cell morphometry

Maurice P. Whelan; Fereydoun Lakestani; Diana Rembges; Maria Grazia Sacco

doi:10.1117/12.728449

17 July 2007 Heterodyne interference microscopy for non-invasive cell morphometry

Maurice P. Whelan, Fereydoun Lakestani, Diana Rembges, Maria Grazia Sacco

Proceedings Volume 6631, Novel Optical Instrumentation for Biomedical Applications III; 66310E (2007) https://doi.org/10.1117/12.728449
Event: European Conferences on Biomedical Optics, 2007, Munich, Germany

Abstract

This paper describes two setups suitable for interference microscopy for high resolution morphological measurements on living cells in culture medium. The first system incorporated a PZT actuator in the reference path of a Mach Zehnder configuration to facilitate digital phase-stepping interferometry. The second system employed two phase-locked acoustooptic modulators to generate a temporal optical carrier to allow a heterodyne approach to phase demodulation. This setup incorporated a digital CMOS camera with full random pixel access which allowed the heterodyne approach to be implemented as a full-field method without any need for electromechanical scanning. The heterodyne approach offers benefits over the phase-stepping method in terms of measurement resolution and speed, typically offering the equivalent of nanometer resolution for cell height measurements with a bandwidth in the order of 200-300 Hz for 1000 pixels. Results for morphological measurements using both systems on red blood cells and keratinocytes are presented.

Citation Download Citation

Maurice P. Whelan, Fereydoun Lakestani, Diana Rembges, and Maria Grazia Sacco "Heterodyne interference microscopy for non-invasive cell morphometry", Proc. SPIE 6631, Novel Optical Instrumentation for Biomedical Applications III, 66310E (17 July 2007); https://doi.org/10.1117/12.728449

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