Real time diffuse reflectance polarisation spectroscopy imaging to evaluate skin microcirculation

Jim O'Doherty; Joakim Henricson; Gert E. Nilsson; Chris Anderson; Martin J. Leahy

doi:10.1117/12.728337

18 July 2007 Real time diffuse reflectance polarisation spectroscopy imaging to evaluate skin microcirculation

Jim O'Doherty, Joakim Henricson, Gert E. Nilsson, Chris Anderson, Martin J. Leahy

Proceedings Volume 6631, Novel Optical Instrumentation for Biomedical Applications III; 66310O (2007) https://doi.org/10.1117/12.728337
Event: European Conferences on Biomedical Optics, 2007, Munich, Germany

Abstract

This article describes the theoretical development and design of a real-time microcirculation imaging system, an extension from a previously technology developed by our group. The technology utilises polarisation spectroscopy, a technique used in order to selectively gate photons returning from various compartments of human skin tissue, namely from the superficial layers of the epidermis, and the deeper backscattered light from the dermal matrix. A consumer-end digital camcorder captures colour data with three individual CCDs, and a custom designed light source consisting of a 24 LED ring light provides broadband illumination over the 400 nm - 700 nm wavelength region. Theory developed leads to an image processing algorithm, the output of which scales linearly with increasing red blood cell (RBC) concentration. Processed images are displayed online in real-time at a rate of 25 frames s^-1, at a frame size of 256 x 256 pixels, and is limited only by computer RAM memory and processing speed. General demonstrations of the technique in vivo display several advantages over similar technology.

Citation Download Citation

Jim O'Doherty, Joakim Henricson, Gert E. Nilsson, Chris Anderson, and Martin J. Leahy "Real time diffuse reflectance polarisation spectroscopy imaging to evaluate skin microcirculation", Proc. SPIE 6631, Novel Optical Instrumentation for Biomedical Applications III, 66310O (18 July 2007); https://doi.org/10.1117/12.728337

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