New perspectives in protein-based biosensors: the glucokinase from <i>B. stearothermophilus</i> and the odorant-binding protein from <i>C. familiaris</i> as probes for non-consuming analyte sensors

Irina M. Kuznetsova; Olga Povarova; Olesya Stepanenko; Konstantin K. Turoverov; Roberta Crescenzo; Antonio Varriale; Maria Staiano; Sabato D'Auria

doi:10.1117/12.753249

27 July 2007 New perspectives in protein-based biosensors: the glucokinase from B. stearothermophilus and the odorant-binding protein from C. familiaris as probes for non-consuming analyte sensors

Irina M. Kuznetsova, Olga Povarova, Olesya Stepanenko, Konstantin K. Turoverov, Roberta Crescenzo, Antonio Varriale, Maria Staiano, Sabato D'Auria

Author Affiliations +

Proceedings Volume 6733, International Conference on Lasers, Applications, and Technologies 2007: Environmental Monitoring and Ecological Applications; Optical Sensors in Biological, Chemical, and Engineering Technologies; and Femtosecond Laser Pulse Filamentation; 673318 (2007) https://doi.org/10.1117/12.753249
Event: International Conference on Lasers, Applications, and Technologies '07, 2007, Minsk, Belarus

Abstract

Glucose sensing and odorant molecules sensing are used as a models to explore the advantages and problems deriving from the use of either enzymes or odorant-binding proteins to develop stable optical biosensors. We report on a novel approach to address the problem of substrate consumption by sensors based on enzymes, namely the utilization of apoenzymes as non-active forms of the protein which are still able to bind the substrate/ligand. We also show studies in which the isolation of an odorant-binding protein from the nose of the dog is used as non-consuming analyte probe for the realization of an integrated optical sensor.

Citation Download Citation

Irina M. Kuznetsova, Olga Povarova, Olesya Stepanenko, Konstantin K. Turoverov, Roberta Crescenzo, Antonio Varriale, Maria Staiano, and Sabato D'Auria "New perspectives in protein-based biosensors: the glucokinase from B. stearothermophilus and the odorant-binding protein from C. familiaris as probes for non-consuming analyte sensors", Proc. SPIE 6733, International Conference on Lasers, Applications, and Technologies 2007: Environmental Monitoring and Ecological Applications; Optical Sensors in Biological, Chemical, and Engineering Technologies; and Femtosecond Laser Pulse Filamentation, 673318 (27 July 2007); https://doi.org/10.1117/12.753249

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