Increased throughput single molecule detection of DNA

Rajan Gurjar; Madhavi Seetamraju; Noah Kolodziejski; Richard Myers; Christopher Staples; James Christian; Michael R Squillante; Gerald Entine

doi:10.1117/12.737323

12 October 2007 Increased throughput single molecule detection of DNA

Rajan Gurjar, Madhavi Seetamraju, Noah Kolodziejski, Richard Myers, Christopher Staples, James Christian, Michael R Squillante, Gerald Entine

Author Affiliations +

Proceedings Volume 6770, Fiber Optic Sensors and Applications V; 677010 (2007) https://doi.org/10.1117/12.737323
Event: Optics East, 2007, Boston, MA, United States

Abstract

In this work, we present research in using confocal optical techniques with femtolitre focal volumes and obtain very high signal-to-noise and signal-to-background ratios for single molecule detection (SMD). We were able to achieve improved signal strength by using highly fluorescent quantum dots and nanopatterned substrates to obtain plasmon induced resonant fluorescence enhancement. A method to simultaneously using multiple excitation spots without the use of confocal apertures and an array of single photon sensitive Geiger mode avalanche photodiodes was used to increase the throughput of the detection system. Using this highly sensitive SMD system, we detect small quantities of synthetic DNA through hybridization eliminating the need of polymerase chain reaction.

Citation Download Citation

Rajan Gurjar, Madhavi Seetamraju, Noah Kolodziejski, Richard Myers, Christopher Staples, James Christian, Michael R Squillante, and Gerald Entine "Increased throughput single molecule detection of DNA", Proc. SPIE 6770, Fiber Optic Sensors and Applications V, 677010 (12 October 2007); https://doi.org/10.1117/12.737323

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available