Multiplexed BioCD for prostate specific antigen detection

Xuefeng Wang; Ming Zhao; David D. Nolte

doi:10.1117/12.759173

12 February 2008 Multiplexed BioCD for prostate specific antigen detection

Xuefeng Wang, Ming Zhao, David D. Nolte

Proceedings Volume 6848, Advanced Biomedical and Clinical Diagnostic Systems VI; 68480S (2008) https://doi.org/10.1117/12.759173
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

Specific protein concentrations in human body fluid can serve as diagnostic markers for some diseases, and a quantitative and high-throughput technique for multiplexed protein detection would speed up diagnosis and facilitate medical research. For this purpose, our group developed the BioCD, a spinning-disc interferometric biosensor on which antibody is immobilized. The detection system adopts a common-path scheme making it ultra stable. The scaling mass sensitivity is below 10 pg/mm for protein surface density. A 25000-spot antibody BioCD was fabricated to measure the concentration of prostate specific antigen (PSA), a protein indicating prostate cancer if its level is high. Statistical analysis of our immunoassay results projects that the detection limit of PSA would reach 20 pg/ml in a 2 mg/ml background solution. For future prospects, a multiplexed BioCD can be produced for simultaneous diagnosis of diverse diseases. For instance, 100 markers above 200 pg/ml could be measured on a single disc given that the detection limit is inversely proportional to square root of the number of spots.

Citation Download Citation

Xuefeng Wang, Ming Zhao, and David D. Nolte "Multiplexed BioCD for prostate specific antigen detection", Proc. SPIE 6848, Advanced Biomedical and Clinical Diagnostic Systems VI, 68480S (12 February 2008); https://doi.org/10.1117/12.759173

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