Corneal imaging by second and third harmonic generation microscopy

Arnaud Brocas; Louis Jay; Eric Mottay; Isabelle Brunette; Tsuneyuki Ozaki

doi:10.1117/12.764432

15 February 2008 Corneal imaging by second and third harmonic generation microscopy

Arnaud Brocas, Louis Jay, Eric Mottay, Isabelle Brunette, Tsuneyuki Ozaki

Proceedings Volume 6860, Multiphoton Microscopy in the Biomedical Sciences VIII; 68600C (2008) https://doi.org/10.1117/12.764432
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

Advanced imaging methods are essential tools for improved outcome of refractive surgery. Second harmonic generation (SHG) and third harmonic generation (THG) microscopy are noninvasive high-resolution imaging methods, which can discriminate the different layers of the cornea, thus having strong impact on the outcome of laser surgery. In this work, we use an Ytterbium femtosecond laser as the laser source, the longer wavelength of which reduces scattering, and allows simultaneous SHG and THG imaging. We present SHG and THG images and profiles of pig corneas that clearly show the anterior surface of the cornea, the entry in the stroma and its end, and the posterior surface of the cornea. These observations allow localizing the epithelium, the stroma and the endothelium. Other experiments give information about the structure and cytology of the corneal layers.

Citation Download Citation

Arnaud Brocas, Louis Jay, Eric Mottay, Isabelle Brunette, and Tsuneyuki Ozaki "Corneal imaging by second and third harmonic generation microscopy", Proc. SPIE 6860, Multiphoton Microscopy in the Biomedical Sciences VIII, 68600C (15 February 2008); https://doi.org/10.1117/12.764432

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