Hybrid photodetector for single-molecule spectroscopy and microscopy

X. Michalet; Adrian Cheng; Joshua Antelman; Motohiro Suyama; Katsushi Arisaka; Shimon Weiss

doi:10.1117/12.763449

21 February 2008 Hybrid photodetector for single-molecule spectroscopy and microscopy

X. Michalet, Adrian Cheng, Joshua Antelman, Motohiro Suyama, Katsushi Arisaka, Shimon Weiss

Proceedings Volume 6862, Single Molecule Spectroscopy and Imaging; 68620F (2008) https://doi.org/10.1117/12.763449
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

We report benchmark tests of a new single-photon counting detector based on a GaAsP photocathode and an electron-bombarded avalanche photodiode developed by Hamamatsu Photonics. We compare its performance with those of standard Geiger-mode avalanche photodiodes. We show its advantages for FCS due to the absence of after-pulsing and for fluorescence lifetime measurements due to its excellent time resolution. Its large sensitive area also greatly simplifies setup alignment. Its spectral sensitivity being similar to that of recently introduced CMOS SPADs, this new detector could become a valuable tool for single-molecule fluorescence measurements, as well as for many other applications.

Citation Download Citation

X. Michalet, Adrian Cheng, Joshua Antelman, Motohiro Suyama, Katsushi Arisaka, and Shimon Weiss "Hybrid photodetector for single-molecule spectroscopy and microscopy", Proc. SPIE 6862, Single Molecule Spectroscopy and Imaging, 68620F (21 February 2008); https://doi.org/10.1117/12.763449

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available