Use of quantum dot-conjugated antibodies to study intracellular cancer biomarkers in living and fixed cells

Jian Ling

doi:10.1117/12.763359

22 February 2008 Use of quantum dot-conjugated antibodies to study intracellular cancer biomarkers in living and fixed cells

Jian Ling

Author Affiliations +

Proceedings Volume 6866, Colloidal Quantum Dots for Biomedical Applications III; 68660T (2008) https://doi.org/10.1117/12.763359
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

Quantum dots have unique properties for long-term immunofluorescence imaging of molecular activities inside living cells. The key is how to deliver the quantum dot-conjugated antibodies into cells and further allow the antibodies freely move inside cells to bind target molecules. This study investigated the feasibility of using Pep-1, a cell penetration protein, to facilitate the internalization of quantum dot-conjugated antibodies for the labeling of two intracellular cervical cancer biomarkers: p16 and Mcm5. Quantum dots were directly conjugated with the antibodies to p16 and Mcm5 and, they were able to stain fixed cells and to differentiate biomarker positive and negative cells. The non-covalent binding between the conjugates and Pep-1 peptides allows the quick internalization of the quantum dot-conjugated antibodies into living cells. The internalized conjugates were concentrated in the perinuclear regions of the biomarker-positive HeLa cells. In the biomarker negative Um-Uc-3 cells, however, the conjugates concentrated in juxtaneclear region. Cells bearing with quantum dots still go through the mitosis process. Although the study indicates many questions need to be answered and many problems need to be solved, the use of cell penetration peptide is a promising method for the intracellular labeling of living cell molecules using quantum dots.

Citation Download Citation

Jian Ling "Use of quantum dot-conjugated antibodies to study intracellular cancer biomarkers in living and fixed cells", Proc. SPIE 6866, Colloidal Quantum Dots for Biomedical Applications III, 68660T (22 February 2008); https://doi.org/10.1117/12.763359

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