The study of sheath flow dark zone phenomenon in dynamic individual cells scattering measurement

Lu Zhang; Hong Zhao; Xiaopin Wang; Weiguang Zhang

doi:10.1117/12.814604

3 October 2008 The study of sheath flow dark zone phenomenon in dynamic individual cells scattering measurement

Lu Zhang, Hong Zhao, Xiaopin Wang, Weiguang Zhang

Proceedings Volume 7155, Ninth International Symposium on Laser Metrology; 71552U (2008) https://doi.org/10.1117/12.814604
Event: Ninth International Symposium on Laser Metrology, 2008, Singapore, Singapore

Abstract

Dynamic cells scattering is one of the most efficient approaches exploring in measurements of cells size, morphology and growth states. This technique can be widely applied in real-time detection for pharmaceutical industry, food industry, liquor industry and other biological fields. A novel method named dynamic individual cells scattering measurement is designed in this paper, which can make cells pass through quartz glass measurement zone one by one with sheath flow driving force. During the experiments, an obvious phenomenon has been found which is called sheath flow dark zone phenomenon (SFDZ). Under the influence of SFDZ, sheath flow forming detection becomes very difficult. In this paper, the causes giving rise to SFDZ have been analyzed. And an improved method is put forward, in which the orifice inside the measurement zone is set as an optical system. Then the illuminating system is redesigned. In this way, almost all the illuminating light can enter orifice so that the total reflection energy decreases substantially. A comparison experiments have been done, which proves the efficiency of this redesigned optical system and its sound effects on SFDZ avoiding.

Citation Download Citation

Lu Zhang, Hong Zhao, Xiaopin Wang, and Weiguang Zhang "The study of sheath flow dark zone phenomenon in dynamic individual cells scattering measurement", Proc. SPIE 7155, Ninth International Symposium on Laser Metrology, 71552U (3 October 2008); https://doi.org/10.1117/12.814604

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KEYWORDS

Scattering

Reflection

Scatter measurement

Signal detection

Laser scattering

Light scattering

Rayleigh scattering

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