Selective cell culture on UV-transparent polymer by F2 laser modification

Y. Hanada; K. Sugioka; H. Kawano; A. Miyawaki; K. Midorikawa

doi:10.1117/12.808815

24 February 2009 Selective cell culture on UV-transparent polymer by F₂ laser modification

Y. Hanada, K. Sugioka, H. Kawano, A. Miyawaki, K. Midorikawa

Proceedings Volume 7201, Laser Applications in Microelectronic and Optoelectronic Manufacturing VII; 72010X (2009) https://doi.org/10.1117/12.808815
Event: SPIE LASE: Lasers and Applications in Science and Engineering, 2009, San Jose, California, United States

Abstract

A microchip made of UV transparent polymer (CYTOP) that can perform selective cell culture has been fabricated by F₂ laser surface modification. The refractive index of CYTOP is almost the same as that of culture medium, which is essential for three dimensional (3D) observation of cells. The F2 laser modification of CYTOP achieves hydrophilicity only on the laser irradiated area with little deterioration of the optical properties and surface smoothness. After the laser modification, HeLa cells were successfully cultured and strongly adhered only on the modified area of CYTOP. The cells patterned on CYTOP were applied for clear 3D observation using an optical microscope in phase contrast mode.

Citation Download Citation

Y. Hanada, K. Sugioka, H. Kawano, A. Miyawaki, and K. Midorikawa "Selective cell culture on UV-transparent polymer by F₂ laser modification", Proc. SPIE 7201, Laser Applications in Microelectronic and Optoelectronic Manufacturing VII, 72010X (24 February 2009); https://doi.org/10.1117/12.808815

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