New developments in two-photon analysis of human skin in vivo

I. Riemann; M. Schwarz; F. Stracke; A. Ehlers; E. Dimitrow; M. Kaatz; K. König; R. Le Harzic

doi:10.1117/12.808770

12 February 2009 New developments in two-photon analysis of human skin in vivo

I. Riemann, M. Schwarz, F. Stracke, A. Ehlers, E. Dimitrow, M. Kaatz, K. König, R. Le Harzic

Proceedings Volume 7203, Commercial and Biomedical Applications of Ultrafast Lasers IX; 720306 (2009) https://doi.org/10.1117/12.808770
Event: SPIE LASE: Lasers and Applications in Science and Engineering, 2009, San Jose, California, United States

Abstract

Two-photon imaging of human skin using ultra short laser pulses can be used to obtain information about the state of cells and tissues by means of their natural autofluorescence. Using this method, it is possible to determine whether the normal cell pattern is disturbed or the autofluorescence is influenced by internal or external stimuli. Two-photon fluorescence lifetime imaging (FLIM) can further enhance this providing information about physiological processes, fluorophores (like NAD(P)H, collagen, keratin, elastin, flavins, melanin,...) and external applied probes inside cells and tissue parts. For example the part of the cells metabolism and energy level can be determined by analyzing the NADH regarding its free / bound state and its oxidized / reduced state. The combination of two-photon imaging with FLIM may lead to a better understanding and diagnosis of skin reactions and disorders. We also present some results of in vivo simultaneous collagen and elastin measurements in skin dermis. Changes of dermal collagen and elastin content are characteristic for skin aging as well as for pathological skin conditions.

Citation Download Citation

I. Riemann, M. Schwarz, F. Stracke, A. Ehlers, E. Dimitrow, M. Kaatz, K. König, and R. Le Harzic "New developments in two-photon analysis of human skin in vivo", Proc. SPIE 7203, Commercial and Biomedical Applications of Ultrafast Lasers IX, 720306 (12 February 2009); https://doi.org/10.1117/12.808770

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
9 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Skin

Tissues

Fluorescence lifetime imaging

In vivo imaging

Collagen

Melanoma

Second-harmonic generation

Show All Keywords

Keywords/Phrases

Search In:

Publication Years