Capillary electrophoresis (CE) peak detection using a wavelet transform technique

Robert Stewart; Andrew Wee; David B. Grayden; Yonggang Zhu

doi:10.1117/12.813449

30 December 2008 Capillary electrophoresis (CE) peak detection using a wavelet transform technique

Robert Stewart, Andrew Wee, David B. Grayden, Yonggang Zhu

Proceedings Volume 7270, Biomedical Applications of Micro- and Nanoengineering IV and Complex Systems; 727012 (2008) https://doi.org/10.1117/12.813449
Event: SPIE Smart Materials, Nano- and Micro-Smart Systems, 2008, Melbourne, Australia

Abstract

Capillary Electrophoresis (CE) is a separation technique that can be used as a sample pre-treatment step in chemical analysis. When coupled with a detection technique, identification of chemical species can be performed on the basis of the elution signals. However, the sensor signals are often complicated by high signal noise, varying baseline and overlapping peaks. There is thus a need for a signal processing technique capable of robustly detecting peaks in acquired sensor data. Here, we report on an algorithm that utilises the Continuous Wavelet Transform (CWT) for the detection of analyte peaks. The algorithm that has been developed makes use of a wavelet equal to the first derivative of a Gaussian function and has been successfully applied to data obtained from a CCD sensor fabricated on a polymer microfluidic separation chip. The algorithm operates by taking the CWT of the sensor response. It then analyses patterns in the local maximum and minimum points evident across scales in the CWT coefficients to find the peaks in the time series data. The performance of two versions of the algorithm have been compared for synthetic data sets each with known baseline, peaks and noise. The improved algorithm has been shown to successfully find peaks with a high sensitivity and low False Discovery Rate within a range of sensitivities.

Citation Download Citation

Robert Stewart, Andrew Wee, David B. Grayden, and Yonggang Zhu "Capillary electrophoresis (CE) peak detection using a wavelet transform technique", Proc. SPIE 7270, Biomedical Applications of Micro- and Nanoengineering IV and Complex Systems, 727012 (30 December 2008); https://doi.org/10.1117/12.813449

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available