Broad-spectrum identification and discrimination between biothreat agents and near-neighbor species

Anthony P. Malanoski; Tomasz A. Leski; Luke Cheng; Zheng Wang; David A. Stenger; Baochuan Lin

doi:10.1117/12.819901

5 May 2009 Broad-spectrum identification and discrimination between biothreat agents and near-neighbor species

Anthony P. Malanoski, Tomasz A. Leski, Luke Cheng, Zheng Wang, David A. Stenger, Baochuan Lin

Proceedings Volume 7306, Optics and Photonics in Global Homeland Security V and Biometric Technology for Human Identification VI; 73060C (2009) https://doi.org/10.1117/12.819901
Event: SPIE Defense, Security, and Sensing, 2009, Orlando, Florida, United States

Abstract

A comprehensive resequencing microarray "Tropical and Emerging Infections (TessArray RPM-TEI 1.0 array)" has been developed to identify and distinguish between biothreat organisms of interest and genetically close related species. This array has undergone validation using an innovative approach where synthetic DNA fragments are used for organisms that it is not safe to work with outside a biosafety 3 facilities. The approach was confirmed from testing a subset of target organisms, such as Ebola viruses and Lassa viruses, at USAMRIID. Most potential biothreat organisms are actually endemic in some part of the world. Proper surveillance of biothreat agents will require some form of monitoring the evolution of the indigenous organisms under their natural environment, so when changes in the organisms occur, the diagnostic assays for these organisms can be reviewed to assure they still provide detection. Using the resequencing microarray (RPM) for detection in locations such as the Africa can support indigenous monitoring as it provides sequence information. An ongoing collaboration with Njala University aims to establish a broad-spectrum pathogen surveillance capability in the Republic of Sierra Leone, West Africa using RPM technology combined with a Geographic Information System. This has the potential to improve the public health efforts in an infected area as well as provide monitoring of the changes occurring to a biothreat organism, i.e. Lassa viruses, in its natural location.

Citation Download Citation

Anthony P. Malanoski, Tomasz A. Leski, Luke Cheng, Zheng Wang, David A. Stenger, and Baochuan Lin "Broad-spectrum identification and discrimination between biothreat agents and near-neighbor species", Proc. SPIE 7306, Optics and Photonics in Global Homeland Security V and Biometric Technology for Human Identification VI, 73060C (5 May 2009); https://doi.org/10.1117/12.819901

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