Efficiency of fluorescence coupling into planar waveguides

R. Bäumner; K. Bodensiek; A. Selle; T. Fricke-Begemann; J. Ihlemann; G. Marowsky

doi:10.1117/12.831579

7 July 2009 Efficiency of fluorescence coupling into planar waveguides

R. Bäumner, K. Bodensiek, A. Selle, T. Fricke-Begemann, J. Ihlemann, G. Marowsky

Proceedings Volume 7368, Clinical and Biomedical Spectroscopy; 736828 (2009) https://doi.org/10.1117/12.831579
Event: European Conferences on Biomedical Optics, 2009, Munich, Germany

Abstract

Using planar waveguides as a platform for optical biosensors allows an efficient and selective fluorescence excitation in close proximity to the waveguide surface. Usually, the fluorescence light that is emitted in the space above the sensor chip is collected and analyzed by suitable free space optics and a detector. Due to the vicinity of the fluorescent molecules to the interface of the waveguide layer, a substantial part of the fluorescence light is coupled back into and collected by the waveguide. The coupling efficiency depends on position, environment and orientation of the molecules. The utilization of this signal for fluorescence detection and analysis can allow a significant simplification of the optical instrumentation. We present a fundamental investigation of the fluorescence collection efficiency into the waveguide by theoretical and experimental means.

Citation Download Citation

R. Bäumner, K. Bodensiek, A. Selle, T. Fricke-Begemann, J. Ihlemann, and G. Marowsky "Efficiency of fluorescence coupling into planar waveguides", Proc. SPIE 7368, Clinical and Biomedical Spectroscopy, 736828 (7 July 2009); https://doi.org/10.1117/12.831579

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