Applying fluorescence microscopy to the investigation of the behavior of foodborne pathogens on produce

Maria T. Brandl

doi:10.1117/12.821816

22 May 2009 Applying fluorescence microscopy to the investigation of the behavior of foodborne pathogens on produce

Maria T. Brandl

Proceedings Volume 7378, Scanning Microscopy 2009; 73782A (2009) https://doi.org/10.1117/12.821816
Event: SPIE Scanning Microscopy, 2009, Monterey, California, United States

Abstract

In the past decade, the development of new tools to better visualize microbes at the cellular scale has spurred a renaissance in the application of microscopy to the study of bacteria in their natural environment. This renewed interest in microscopy may be largely attributable to the advent of the confocal laser scanning microscope (CLSM) and to the discovery of the green fluorescent protein. This article provides information about the use of fluorescence microscopy combined with fluorescent labels such as GFP, DsRed, and DNA stains, with immunofluorescence, and with digital image analysis, to examine the behavior of bacteria and other microbes on plant surfaces. Some of the advantages and pitfalls of these methods will be described using practical examples derived from studies of the ecology of foodborne pathogens, namely Salmonella enterica and E. coli O157:H7, on fresh fruit and vegetables. Confocal microscopy has been a powerful approach to uncover some of the factors involved in the association of produce with epidemics caused by these human pathogens and their interaction with other microbes in their nonhost environment.

Citation Download Citation

Maria T. Brandl "Applying fluorescence microscopy to the investigation of the behavior of foodborne pathogens on produce", Proc. SPIE 7378, Scanning Microscopy 2009, 73782A (22 May 2009); https://doi.org/10.1117/12.821816

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