The utilization of a non-invasive fluorescence imaging system to follow clinical dermatological MAL-PDT

Jessica Tyrrell; Sandra Campbell; Alison Curnow

doi:10.1117/12.822756

13 July 2009 The utilization of a non-invasive fluorescence imaging system to follow clinical dermatological MAL-PDT

Jessica Tyrrell, Sandra Campbell, Alison Curnow

Proceedings Volume 7380, Photodynamic Therapy: Back to the Future; 73801P (2009) https://doi.org/10.1117/12.822756
Event: 12th World Congress of the International Photodynamic Association, 2009, Seattle, Washington, United States

Abstract

This study employed a commercially available, non-invasive, fluorescence imaging system (Dyaderm, Biocam, Germany), to measure protoporphyrin IX (PpIX) concentration at several different stages during clinical dermatological methyl aminolevulinate photodynamic therapy (MAL-PDT). We validated the system prior to use to ensure that the PpIX changes witnessed were accurate and not due to environmental or user induced artifacts. The system was then employed to acquire color (morphological) and fluorescent (physiological) images simultaneously during dermatological PDT. Clinical data was collected from a range of licensed dermatological conditions (actinic keratosis, Bowen's disease and superficial basal cell carcinoma) during initial and subsequent PDT treatment cycles. The initial clinical data indicated that each type of licensed lesion considered responded in a similar manner following the application of Metvix (Galderma, U.K.) and the subsequent light irradiation (Aktilite, Galderma, U.K.). Images acquired three hours after Metvix application showed a significant increase in PpIX concentration within the lesion (P < 0.05), whilst PpIX levels in the surrounding normal tissue remained unaltered. After irradiation, the PpIX concentration was significantly decreased and returned to a level similar to the initial concentration originally observed. Lesions that received subsequent treatment cycles accumulated significantly less PpIX (P < 0.05) prior to irradiation.

Citation Download Citation

Jessica Tyrrell, Sandra Campbell, and Alison Curnow "The utilization of a non-invasive fluorescence imaging system to follow clinical dermatological MAL-PDT", Proc. SPIE 7380, Photodynamic Therapy: Back to the Future, 73801P (13 July 2009); https://doi.org/10.1117/12.822756

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