A 2 Ghz Frequency-Domain Fluorometer; Picosecond Resolution Of Protein Fluorescence And Anisotropy Decays

Joseph R. Lakowicz; Gabor Laczko; Ignacy Gryczynski; Henryk Cherek; N. Joshi; M. L. Johnson

doi:10.1117/12.966918

1 January 1987 A 2 Ghz Frequency-Domain Fluorometer; Picosecond Resolution Of Protein Fluorescence And Anisotropy Decays

Joseph R. Lakowicz, Gabor Laczko, Ignacy Gryczynski, Henryk Cherek, N. Joshi, M. L. Johnson

Author Affiliations +

Proceedings Volume 0743, Fluorescence Detection; (1987) https://doi.org/10.1117/12.966918
Event: OE LASE'87 and EO Imaging Symposium, 1987, Los Angeles, CA, United States

Abstract

We developed a frequency-domain fluorometer which operates from 4 to 2000 MHz. The modulated excitation is provided by the harmonic content of a laser pulse train (7.59 MHz, 5 psec) from a syncronously pumped and cavity dumped dye laser. The phase angle and modulation of the emission are measured with a microchannel plate photomultiplier. Cross-correlation detection is performed outside the PMT. The performance was verified by measurement of known time delays and examination of standard fluorophores. The detector displayed no detectable color effect, with the 300 to 600 nm difference being less than 5 psec. The precision of the measurements is adequate to detect differences of 20 psec for decay times of 500 ps.

Citation Download Citation

Joseph R. Lakowicz, Gabor Laczko, Ignacy Gryczynski, Henryk Cherek, N. Joshi, and M. L. Johnson "A 2 Ghz Frequency-Domain Fluorometer; Picosecond Resolution Of Protein Fluorescence And Anisotropy Decays", Proc. SPIE 0743, Fluorescence Detection, (1 January 1987); https://doi.org/10.1117/12.966918

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available