Detection system of capillary array electrophoresis microchip based on optical fiber

Xiaobo Yang; Haiming Bai; Weiping Yan

doi:10.1117/12.837852

20 November 2009 Detection system of capillary array electrophoresis microchip based on optical fiber

Xiaobo Yang, Haiming Bai, Weiping Yan

Proceedings Volume 7510, 2009 International Conference on Optical Instruments and Technology: MEMS/NEMS Technology and Applications; 751006 (2009) https://doi.org/10.1117/12.837852
Event: International Conference on Optical Instrumentation and Technology, 2009, Shanghai, China

Abstract

To meet the demands of the post-genomic era study and the large parallel detections of epidemic diseases and drug screening, the high throughput micro-fluidic detection system is needed urgently. A scanning laser induced fluorescence detection system based on optical fiber has been established by using a green laser diode double-pumped solid-state laser as excitation source. It includes laser induced fluorescence detection subsystem, capillary array electrophoresis micro-chip, channel identification unit and fluorescent signal processing subsystem. V-shaped detecting probe composed with two optical fibers for transmitting the excitation light and detecting induced fluorescence were constructed. Parallel four-channel signal analysis of capillary electrophoresis was performed on this system by using Rhodamine B as the sample. The distinction of different samples and separation of samples were achieved with the constructed detection system. The lowest detected concentration is 1×10^-5 mol/L for Rhodamine B. The results show that the detection system possesses some advantages, such as compact structure, better stability and higher sensitivity, which are beneficial to the development of microminiaturization and integration of capillary array electrophoresis chip.

Citation Download Citation

Xiaobo Yang, Haiming Bai, and Weiping Yan "Detection system of capillary array electrophoresis microchip based on optical fiber", Proc. SPIE 7510, 2009 International Conference on Optical Instruments and Technology: MEMS/NEMS Technology and Applications, 751006 (20 November 2009); https://doi.org/10.1117/12.837852

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