Tomographic imaging system using digital holographic technique based on integrating four buckets phase shifting interferometry

Gihyeon Min; Ju Wan Kim; Woo Jun Choi; Eun Seo Choi; Byeong Ha Lee

doi:10.1117/12.841683

26 February 2010 Tomographic imaging system using digital holographic technique based on integrating four buckets phase shifting interferometry

Gihyeon Min, Ju Wan Kim, Woo Jun Choi, Eun Seo Choi, Byeong Ha Lee

Author Affiliations +

Proceedings Volume 7568, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII; 756824 (2010) https://doi.org/10.1117/12.841683
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

We present a tomographic imaging system that is applied a continuous phase shifting interferometry scheme to digital holographic microscopy (DHM). The proposed scheme achieves en-face tomographic image from digitally recorded original hologram by 2-D sensor array. Although images obtained at out-of-focus position, the application of integrating four bucket technique to digital hologram produces refocused en-face image with showing clear field of view. The proposed technique has advantages such as reduced phase errors and faster acquisition speed when it compared with conventional discrete phase stepping method. The performance of the system is demonstrated with presenting of the images on a scratched mirror surface and of an USAF resolution target. The reconstructed images are compared with conventional microscopic images, which reveal good aggrements. We believe that the proposed method enables tomographic imaging of biological samples with providing reduced noise level and improved imaging speed.

Citation Download Citation

Gihyeon Min, Ju Wan Kim, Woo Jun Choi, Eun Seo Choi, and Byeong Ha Lee "Tomographic imaging system using digital holographic technique based on integrating four buckets phase shifting interferometry", Proc. SPIE 7568, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VIII, 756824 (26 February 2010); https://doi.org/10.1117/12.841683

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