Synthesis and surface modification of highly fluorescent gold nanoclusters and their exploitation for cellular labeling

Cheng-An J. Lin; Chih-Hsien Lee; Jiun-Tai Hsieh; Wan-Chun Yu; Hong-Zhi Yang; Jimmy K. Li; Ralph Sperling; Hsueh-Hsiao Wang; Hung-I Yeh; Wolfgang J. Parak; Walter H. Chang

doi:10.1117/12.841540

17 February 2010 Synthesis and surface modification of highly fluorescent gold nanoclusters and their exploitation for cellular labeling

Cheng-An J. Lin, Chih-Hsien Lee, Jiun-Tai Hsieh, Wan-Chun Yu, Hong-Zhi Yang, Jimmy K. Li, Ralph Sperling, Hsueh-Hsiao Wang, Hung-I Yeh, Wolfgang J. Parak, Walter H. Chang

Author Affiliations +

Proceedings Volume 7575, Colloidal Quantum Dots for Biomedical Applications V; 757506 (2010) https://doi.org/10.1117/12.841540
Event: SPIE BiOS, 2010, San Francisco, California, United States

Abstract

We introduce a general approach to make colorful fluorescent gold nanoclusters which are protected by dihydrolipoic acid, mercaptoundecanoic acid and polyethylenimine. The fluorescent gold nanoclusters can perform a variety of bioconjugation processes such as PEGylation, biotinylation as well as forming complex nanobioconjugates with streptavidins. The brightening effects under proper surface modification are also reported. The clusters have a decent quantum yield, high colloidal stability, and can be readily conjugated with biological molecules. Nonspecific uptake by human aortic endothelial cells is demonstrated.

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Cheng-An J. Lin, Chih-Hsien Lee, Jiun-Tai Hsieh, Wan-Chun Yu, Hong-Zhi Yang, Jimmy K. Li, Ralph Sperling, Hsueh-Hsiao Wang, Hung-I Yeh, Wolfgang J. Parak, and Walter H. Chang "Synthesis and surface modification of highly fluorescent gold nanoclusters and their exploitation for cellular labeling", Proc. SPIE 7575, Colloidal Quantum Dots for Biomedical Applications V, 757506 (17 February 2010); https://doi.org/10.1117/12.841540

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