Preparation and properties of new complex sensing film for fiber optic glucose sensor

Jun Huang; Chao Wang; Yinquan Yuan; Hai Wang; Liyun Ding; Dian Fan

doi:10.1117/12.849379

24 April 2010 Preparation and properties of new complex sensing film for fiber optic glucose sensor

Jun Huang, Chao Wang, Yinquan Yuan, Hai Wang, Liyun Ding, Dian Fan

Proceedings Volume 7673, Advanced Environmental, Chemical, and Biological Sensing Technologies VII; 76730X (2010) https://doi.org/10.1117/12.849379
Event: SPIE Defense, Security, and Sensing, 2010, Orlando, Florida, United States

Abstract

In this paper, a new complex sensing film containing both optical indicator and enzyme was prepared and its sensing properties were studied, using cellulose acetate (CA) as the carrier and tris (2,2'-blpyridyl) dichloro-ruthenium (II) hexahydrate (Ru(bpy)₃Cl₂) as the indicator. The cross-linking method was used to immobilize glucose oxidase (GOD). The immobilization conditions were optimized: the concentration of sodium periodate as 0.2 M and the reaction time as 30 min, those for ethanediamine as 0.03 M and 2.5 hours, those for GA as 1.5% (v/v) and 2 hours, those for GOD as 35 mg/ml and 21h. The optimal temperature and pH value for the catalytic properties of the sensing film are 38 °C and 7.0, respectively. A fiber optic glucose sensor with this complex sensing film has been studied. The results show that its detecting range is 100-600 mg/dl and its response time is less than 20 seconds.

Citation Download Citation

Jun Huang, Chao Wang, Yinquan Yuan, Hai Wang, Liyun Ding, and Dian Fan "Preparation and properties of new complex sensing film for fiber optic glucose sensor", Proc. SPIE 7673, Advanced Environmental, Chemical, and Biological Sensing Technologies VII, 76730X (24 April 2010); https://doi.org/10.1117/12.849379

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