DNA recognition by peptide nucleic acid-modified PCFs: from models to real samples

S. Selleri; E. Coscelli; F. Poli; D. Passaro; A. Cucinotta; C. Lantano; R. Corradini; R. Marchelli

doi:10.1117/12.854355

17 May 2010 DNA recognition by peptide nucleic acid-modified PCFs: from models to real samples

S. Selleri, E. Coscelli, F. Poli, D. Passaro, A. Cucinotta, C. Lantano, R. Corradini, R. Marchelli

Proceedings Volume 7715, Biophotonics: Photonic Solutions for Better Health Care II; 77151E (2010) https://doi.org/10.1117/12.854355
Event: SPIE Photonics Europe, 2010, Brussels, Belgium

Abstract

The increased concern, emerged in the last few years, on food products safety has stimulated the research on new techniques for traceability of raw food materials. DNA analysis is one of the most powerful tools for the certification of food quality, and it is presently performed through the polymerase chain reaction technique. Photonic crystal fibers, due to the presence of an array of air holes running along their length, can be exploited for performing DNA recognition by derivatizing hole surfaces and checking hybridization of complementary nucledotide chains in the sample. In this paper the application of a suspended core photonic crystal fiber in the recognition of DNA sequences is discussed. The fiber is characterized in terms of electromagnetic properties by means of a full-vector modal solver based on the finite element method. Then, the performances of the fiber in the recognition of mall synthetic oligonucleotides are discussed, together with a test of the possibility to extend this recognition to samples of DNA of applicative interest, such as olive leaves.

Citation Download Citation

S. Selleri, E. Coscelli, F. Poli, D. Passaro, A. Cucinotta, C. Lantano, R. Corradini, and R. Marchelli "DNA recognition by peptide nucleic acid-modified PCFs: from models to real samples", Proc. SPIE 7715, Biophotonics: Photonic Solutions for Better Health Care II, 77151E (17 May 2010); https://doi.org/10.1117/12.854355

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