Measuring binding kinetics of biomolecular interactions using a localized surface plasmon couple fluorescence fiber optic biosensor

Ying-Feng Chang; Jo-Ping Hsieh; Li-Chen Su; Ying-Chang Li; Cheng-Chung Lee; Chien Chou

doi:10.1117/12.863621

24 August 2010 Measuring binding kinetics of biomolecular interactions using a localized surface plasmon couple fluorescence fiber optic biosensor

Ying-Feng Chang, Jo-Ping Hsieh, Li-Chen Su, Ying-Chang Li, Cheng-Chung Lee, Chien Chou

Author Affiliations +

Proceedings Volume 7759, Biosensing III; 77590H (2010) https://doi.org/10.1117/12.863621
Event: SPIE NanoScience + Engineering, 2010, San Diego, California, United States

Abstract

In this study, we describe a novel method for analyzing protein-protein binding kinetics at ultra-low concentration (1 pg/mL) using a localized surface plasmon coupled fluorescence fiber-optic biosensor (LSPCF-FOB). The association and dissociation rate constants, ka and kd, respectively, for the binding kinetics of the mouse IgG/ anti-mouse IgG interaction have been calculated to be ka = (5.9928±3.1540)x10⁶ M^-1s^-1 and kd = (1.0587±0.5572)x10^-3 s^-1. The theoretical basis of this analytical approach is a rapid-mixing model integrated with a two-compartment model; has been experimentally verified in this study as well. The LSPCF-FOB provides a potentially alternative option for characterizing the interaction of biomolecules at ultra-low concentrations.

Citation Download Citation

Ying-Feng Chang, Jo-Ping Hsieh, Li-Chen Su, Ying-Chang Li, Cheng-Chung Lee, and Chien Chou "Measuring binding kinetics of biomolecular interactions using a localized surface plasmon couple fluorescence fiber optic biosensor", Proc. SPIE 7759, Biosensing III, 77590H (24 August 2010); https://doi.org/10.1117/12.863621

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