Microfluidic device for capture and isolation of single cells

Alexander P. Hsiao; Kristopher D. Barbee; Xiaohua Huang

doi:10.1117/12.861563

24 August 2010 Microfluidic device for capture and isolation of single cells

Alexander P. Hsiao, Kristopher D. Barbee, Xiaohua Huang

Proceedings Volume 7759, Biosensing III; 77590W (2010) https://doi.org/10.1117/12.861563
Event: SPIE NanoScience + Engineering, 2010, San Diego, California, United States

Abstract

We describe a microfluidic device capable of trapping, isolating, and lysing individual cells in parallel using dielectrophoretic forces and a system of PDMS channels and valves. The device consists of a glass substrate patterned with electrodes and two PDMS layers comprising of the microfluidic channels and valve control channels. Individual cells are captured by positive dielectrophoresis using the microfabricated electrode pairs. The cells are then isolated into nanoliter compartments using pneumatically actuated PDMS valves. Following isolation, the cells are lysed open by applying an electric field using the same electrode pairs. With the ability to capture and compartmentalize single cells our device may be combined with analytical methods for in situ molecular analysis of cellular components from single cells in a highly parallel manner.

Citation Download Citation

Alexander P. Hsiao, Kristopher D. Barbee, and Xiaohua Huang "Microfluidic device for capture and isolation of single cells", Proc. SPIE 7759, Biosensing III, 77590W (24 August 2010); https://doi.org/10.1117/12.861563

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