Measuring the diffusion of fluorophores in human skin by two-photon fluorescence correlation spectroscopy combined with measurements of point spread function

Stina Guldbrand; Carl Simonsson; Mattias Goksör; Maria Smedh; Marica B. Ericson

doi:10.1117/12.874191

3 March 2011 Measuring the diffusion of fluorophores in human skin by two-photon fluorescence correlation spectroscopy combined with measurements of point spread function

Stina Guldbrand, Carl Simonsson, Mattias Goksör, Maria Smedh, Marica B. Ericson

Author Affiliations +

Proceedings Volume 7903, Multiphoton Microscopy in the Biomedical Sciences XI; 790329 (2011) https://doi.org/10.1117/12.874191
Event: SPIE BiOS, 2011, San Francisco, California, United States

Abstract

Two-photon excitation fluorescence correlation spectroscopy (TPFCS) has been used in combination with measurements of the point spread function (PSF), for quantitative analysis of fluorophores in excised human skin. Measurements have been performed at depths between 0 and 40 μm. The PSF, measured as full width at half maximum, was found not to depend on the depth. Measurements revealed difference in diffusion coefficient depending on extra- or intracellular location of fluorophore. The number of molecules was accumulating close to the surface and then decreased by the depth. The results from our study show that TPFCS can be used for quantitative analyses of fluorescent compounds in human skin.

Citation Download Citation

Stina Guldbrand, Carl Simonsson, Mattias Goksör, Maria Smedh, and Marica B. Ericson "Measuring the diffusion of fluorophores in human skin by two-photon fluorescence correlation spectroscopy combined with measurements of point spread function", Proc. SPIE 7903, Multiphoton Microscopy in the Biomedical Sciences XI, 790329 (3 March 2011); https://doi.org/10.1117/12.874191

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