Confocal Scanning Laser Fluorescence And Reflection Microscopy: Measurements Of The 3-D Image Formation And Applications In Biology

H. T.M. van der Voort; G. J. Brakenhoff; C.G. A.M. Janssen; J. A.C. Valkenburg; N. Nanninga

doi:10.1117/12.941510

3 August 1987 Confocal Scanning Laser Fluorescence And Reflection Microscopy: Measurements Of The 3-D Image Formation And Applications In Biology

H. T.M. van der Voort, G. J. Brakenhoff, C.G. A.M. Janssen, J. A.C. Valkenburg, N. Nanninga

Author Affiliations +

Proceedings Volume 0809, Scanning Imaging Technology; (1987) https://doi.org/10.1117/12.941510
Event: Fourth International Symposium on Optical and Optoelectronic Applied Sciences and Engineering, 1987, The Hague, Netherlands

Abstract

A confocal scanning laser microscope of the on-axis type, directly coupled to an image processing system is described. Results of measurements of the actual response functions, both in fluorescence and reflection are presented. Applications of the confocal technique in the area of biology in combination with image processing are demonstrated.

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H. T.M. van der Voort, G. J. Brakenhoff, C.G. A.M. Janssen, J. A.C. Valkenburg, and N. Nanninga "Confocal Scanning Laser Fluorescence And Reflection Microscopy: Measurements Of The 3-D Image Formation And Applications In Biology", Proc. SPIE 0809, Scanning Imaging Technology, (3 August 1987); https://doi.org/10.1117/12.941510

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