Novel, rapid DNA-based on-chip bacterial identification system combining dielectrophoresis and amplification-free fluorescent resonance energy transfer assisted in-situ hybridization (FRET-ISH)

Michelle M. Packard; Maxim Shusteff; Evangelyn Alocilja

doi:10.1117/12.892425

12 September 2011 Novel, rapid DNA-based on-chip bacterial identification system combining dielectrophoresis and amplification-free fluorescent resonance energy transfer assisted in-situ hybridization (FRET-ISH)

Michelle M. Packard, Maxim Shusteff, Evangelyn Alocilja

Author Affiliations +

Proceedings Volume 8099, Biosensing and Nanomedicine IV; 80990J (2011) https://doi.org/10.1117/12.892425
Event: SPIE NanoScience + Engineering, 2011, San Diego, California, United States

Abstract

Although real-time PCR (RT-PCR) has become a diagnostic standard for rapid identification of bacterial species, typical methods remain time-intensive due to sample preparation and amplification cycle times. The assay described in this work incorporates on-chip dielectrophoretic capture and concentration of bacterial cells, thermal lysis, cell permeabilization, and nucleic acid denaturation and fluorescence resonance energy transfer assisted in-situ hybridization (FRET-ISH) species identification. Identification is achieved completely on chip in less than thirty minutes from receipt of sample compared to multiple hours required by traditional RT-PCR and its requisite sample preparation.

Citation Download Citation

Michelle M. Packard, Maxim Shusteff, and Evangelyn Alocilja "Novel, rapid DNA-based on-chip bacterial identification system combining dielectrophoresis and amplification-free fluorescent resonance energy transfer assisted in-situ hybridization (FRET-ISH)", Proc. SPIE 8099, Biosensing and Nanomedicine IV, 80990J (12 September 2011); https://doi.org/10.1117/12.892425

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