Microspot surface enhanced fluorescence from sculptured thin films for control of antibody immobilization

Alina Karabchevsky; Christian Patzig; Bernd Rauschenbach; Ibrahim Abdulhalim

doi:10.1117/12.899616

26 September 2011 Microspot surface enhanced fluorescence from sculptured thin films for control of antibody immobilization

Alina Karabchevsky, Christian Patzig, Bernd Rauschenbach, Ibrahim Abdulhalim

Author Affiliations +

Proceedings Volume 8104, Nanostructured Thin Films IV; 81040L (2011) https://doi.org/10.1117/12.899616
Event: SPIE NanoScience + Engineering, 2011, San Diego, California, United States

Abstract

Nano-sculptured thin films (STF) are prepared by the glancing angle deposition technique and take different forms of nano columnar structures. Varieties of STFs are investigated to find the optimum structure for biosensing based on the surface enhanced fluourescence (SEF). The highest amplification of fluorescent signal is found for Ag based STFs on fused silica giving an enhancement factor of x23°, where h=400nm, d=75nm, á=23o relative to Ag dense film using fluorescent dye Rhodamine 123. Based on this, a demonstration of monitoring of antibodies and even confirmation of successful immobilization of the receptors presented. Bound antibody to the thiol self assembly monolayer on sample surface is then quantified by means of the fluorescent signal. Upon excitation of the fluorophore by Hg source light, a CCD camera with a controlled exposure time detects the pattern of fluorescent antibody/E-coli bacteria colonies on the STF surface. A fiber optic holder attached to the microscope allowed quantitative measurement of the fluorescence spectrum on a microspot.

Citation Download Citation

Alina Karabchevsky, Christian Patzig, Bernd Rauschenbach, and Ibrahim Abdulhalim "Microspot surface enhanced fluorescence from sculptured thin films for control of antibody immobilization", Proc. SPIE 8104, Nanostructured Thin Films IV, 81040L (26 September 2011); https://doi.org/10.1117/12.899616

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available