Effect of non-homogenous thermal stress during sub-lethal photodynamic antimicrobial chemotherapy

N. Gadura; D. Kokkinos; S. Dehipawala; E. Cheung; R. Sullivan; R. Subramaniam; P. Schneider; G. Tremberger Jr.; T. Holden; D. Lieberman; T. Cheung

doi:10.1117/12.909486

9 March 2012 Effect of non-homogenous thermal stress during sub-lethal photodynamic antimicrobial chemotherapy

N. Gadura, D. Kokkinos, S. Dehipawala, E. Cheung, R. Sullivan, R. Subramaniam, P. Schneider, G. Tremberger Jr., T. Holden, D. Lieberman, T. Cheung

Author Affiliations +

Proceedings Volume 8211, Mechanisms for Low-Light Therapy VII; 82110O (2012) https://doi.org/10.1117/12.909486
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

Pathogens could be inactivated via a light source coupled with a photosensitizing agent in photodynamic antimicrobial chemotherapy (PACT). This project studied the effect of non-homogenous substrate on cell colony. The non-homogeneity could be controlled by iron oxide nano-particles doping in porous glassy substrates such that each cell would experience tens of hot spots when illuminated with additional light source. The substrate non-homogeneity was characterized by Atomic Force Microscopy, Transmission Electron Microscopy and Extended X-Ray Absorption Fine Structure at Brookhaven Synchrotron Light Source. Microscopy images of cell motion were used to study the motility. Laboratory cell colonies on non-homogenous substrates exhibit reduced motility similar to those observed with sub-lethal PCAT treatment. Such motility reduction on non-homogenous substrate is interpreted as the presence of thermal stress. The studied pathogens included E. coli and Pseudomonas aeruginosa. Non-pathogenic microbes Bacillus subtilis was also studied for comparison. The results show that sub-lethal PACT could be effective with additional non-homogenous thermal stress. The use of non-uniform illumination on a homogeneous substrate to create thermal stress in sub-micron length scale is discussed via light correlation in propagation through random medium. Extension to sub-lethal PACT application complemented with thermal stress would be an appropriate application.

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N. Gadura, D. Kokkinos, S. Dehipawala, E. Cheung, R. Sullivan, R. Subramaniam, P. Schneider, G. Tremberger Jr., T. Holden, D. Lieberman, and T. Cheung "Effect of non-homogenous thermal stress during sub-lethal photodynamic antimicrobial chemotherapy", Proc. SPIE 8211, Mechanisms for Low-Light Therapy VII, 82110O (9 March 2012); https://doi.org/10.1117/12.909486

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KEYWORDS

Fractal analysis

Light sources

Photoacoustic tomography

Thermal effects

Pathogens

Photodynamic therapy

Microscopy

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