Development of multimodal microscope combined with confocal imaging and two-photon imaging

Wanhee Chun; Dukho Do; Dae-Gab Gweon

doi:10.1117/12.907696

29 February 2012 Development of multimodal microscope combined with confocal imaging and two-photon imaging

Wanhee Chun, Dukho Do, Dae-Gab Gweon

Proceedings Volume 8216, Multimodal Biomedical Imaging VII; 821611 (2012) https://doi.org/10.1117/12.907696
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

Multimodal microscope which obtains various images for the same interest area simultaneously plays an important role in the process of comprehensive analysis for biological information. Confocal imaging for reflection and fluorescence emission light and two-photon imaging dealing with nonlinear optical effect are operated in the unified platform by sharing system configuration commonly used for those imaging. Reflection light, fluorescence light and nonlinear optical signal from the same focal point are detected through separate channels and converted to respective images. Common optical system is especially customized to satisfy their requirements considering wavelength band of light used for imaging. Multimodal microscope is implemented and verified through multichannel images for bioexperiments. Confocal reflection imaging for label-free specimen, confocal fluorescence imaging and two-photon fluorescence imaging for specifically stained target are realized and make complementary analysis. The used light signals, continuous wave light from NUV to NIR and pulsed light, is verified through imaging results of designed multimodal microscope.

Citation Download Citation

Wanhee Chun, Dukho Do, and Dae-Gab Gweon "Development of multimodal microscope combined with confocal imaging and two-photon imaging", Proc. SPIE 8216, Multimodal Biomedical Imaging VII, 821611 (29 February 2012); https://doi.org/10.1117/12.907696

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KEYWORDS

Luminescence

Confocal microscopy

Microscopes

Two photon imaging

Imaging systems

Pulsed laser operation

Reflectivity

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