An analysis of the influences of biological variance, measurement error, and uncertainty on retinal photothermal damage threshold studies

David A. Wooddell Jr.; Christine M. Schubert-Kabban; Raymond R. Hill

doi:10.1117/12.914645

9 February 2012 An analysis of the influences of biological variance, measurement error, and uncertainty on retinal photothermal damage threshold studies

David A. Wooddell Jr., Christine M. Schubert-Kabban, Raymond R. Hill

Author Affiliations +

Proceedings Volume 8221, Optical Interactions with Tissue and Cells XXIII; 822114 (2012) https://doi.org/10.1117/12.914645
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

Safe exposure limits for directed energy sources are derived from a compilation of known injury thresholds taken primarily from animal models and simulation data. The summary statistics for these experiments are given as exposure levels representing a 50% probability of injury, or ED50, and associated variance. We examine biological variance in focal geometries and thermal properties and the influence each has in singlepulse ED50 threshold studies for 514-, 694-, and 1064-nanometer laser exposures in the thermal damage time domain. Damage threshold is defined to be the amount of energy required for a retinal burn on at least one retinal pigment epithelium (RPE) cell measuring approximately 10 microns in diameter. Better understanding of experimental variance will allow for more accurate safety buffers for exposure limits and improve directed energy research methodology.

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David A. Wooddell Jr., Christine M. Schubert-Kabban, and Raymond R. Hill "An analysis of the influences of biological variance, measurement error, and uncertainty on retinal photothermal damage threshold studies", Proc. SPIE 8221, Optical Interactions with Tissue and Cells XXIII, 822114 (9 February 2012); https://doi.org/10.1117/12.914645

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