Investigating the effects of terahertz radiation on Bacillus subtilis

Jillian P. Giles; Brittany J. Raitt; Cecil S. Joseph; Mark E. Hines; Robert H. Giles

doi:10.1117/12.910724

9 February 2012 Investigating the effects of terahertz radiation on Bacillus subtilis

Jillian P. Giles, Brittany J. Raitt, Cecil S. Joseph, Mark E. Hines, Robert H. Giles

Proceedings Volume 8222, Dynamics and Fluctuations in Biomedical Photonics IX; 822213 (2012) https://doi.org/10.1117/12.910724
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

Medical and security sensing applications of Terahertz (THz) imaging are currently being developed. As a result, there is a need to further investigate the effects of THz radiation on biological systems. In this study, a 94 GHz mechanically tuned Gunn Oscillator was used to irradiate Bacillus subtilis at 94 GHz. The bacteria were cultured in trypticase soy broth (TSB) and placed in polystyrene 96 well plates. The samples where irradiated during the exponential growth phase for 1, 2, and 24 hours. Both the experimental and control plates were kept at room temperature (~25°C) and were monitored for the duration of the experiment using thermocouples interfaced with a computer via Labview software. By evaluating the absorption of each well at 600nm immediately before and after irradiation, the population density within each well was assessed. Following this, the metabolic activity of each well was measured after irradiation by adding tetrazolium dye, XTT, to the wells and evaluating the absorption of each well at 490nm after 2 hours of incubation.

Citation Download Citation

Jillian P. Giles, Brittany J. Raitt, Cecil S. Joseph, Mark E. Hines, and Robert H. Giles "Investigating the effects of terahertz radiation on Bacillus subtilis", Proc. SPIE 8222, Dynamics and Fluctuations in Biomedical Photonics IX, 822213 (9 February 2012); https://doi.org/10.1117/12.910724

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