Circulation times of cancer cells by in vivo flow cytometry

Li Zhang; Yan Li; Zhengqin Gu; Tong Chen; Cheng Wang; Xunbin Wei

doi:10.1117/12.911161

14 February 2012 Circulation times of cancer cells by in vivo flow cytometry

Li Zhang, Yan Li, Zhengqin Gu, Tong Chen, Cheng Wang, Xunbin Wei

Proceedings Volume 8224, Biophotonics and Immune Responses VII; 82240B (2012) https://doi.org/10.1117/12.911161
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

Liver cancer is one of the most common malignancies in the world, with approximately 1,000,000 cases reported every year. Hepatocellular carcinoma may metastasize to lung, bones, kidney, and many other organs. Surgical resection, liver transplantation, chemotherapy and radiation therapy are the foundation of current HCC therapies. However the outcomes are poor: the survival rate is almost zero for metastatic HCC patients. Molecular mechanisms of HCC metastasis need to be understood better and new therapies must be developed. A recently developed "in vivo flow cytometer" combined with real-time confocal fluorescence imaging are used to assess spreading and the circulation kinetics of liver tumor cells. The in vivo flow cytometer has the capability to detect and quantify continuously the number and flow characteristics of fluorescently labeled cells in vivo in real time without extracting blood sample. We have measured the depletion kinetics of two related human HCC cell lines, high-metastatic HCCLM3 cells and low-metastatic HepG2 cells, which were from the same origin and obtained by repetitive screenings in mice. >60% HCCLM3 cells are depleted within the first hour. Interestingly, the low-metastatic HepG2 cells possess noticeably slower depletion kinetics. In comparison, <40% HepG2 cells are depleted within the first hour. The differences in depletion kinetics might provide insights into early metastasis processes.

Citation Download Citation

Li Zhang, Yan Li, Zhengqin Gu, Tong Chen, Cheng Wang, and Xunbin Wei "Circulation times of cancer cells by in vivo flow cytometry", Proc. SPIE 8224, Biophotonics and Immune Responses VII, 82240B (14 February 2012); https://doi.org/10.1117/12.911161

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