Full-field OCT combined with optical tweezer

Woo June Choi; Kwan Seob Park; Tae Joong Eom; Myung-Kyu Oh; Byeong-Ha Lee

doi:10.1117/12.908380

2 February 2012 Full-field OCT combined with optical tweezer

Woo June Choi, Kwan Seob Park, Tae Joong Eom, Myung-Kyu Oh, Byeong-Ha Lee

Proceedings Volume 8227, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIX; 82271J (2012) https://doi.org/10.1117/12.908380
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

We present an optical tweezer technique assisted full-field optical coherence tomography (FF-OCT) system. The proposed scheme enables ultrahigh-resolution OCT imaging of a floating object optically trapped by single-beam gradient force in medium. The set up consists of a Linnik type of white light interference microscope combined with an optical tweezer system. The optical trap is formed by tightly focusing a 1064 nm Q-switching pulsed laser beam with a microscope objective lens of high numerical aperture (1.0 NA) in sample arm of the OCT interferometer. This co-sharing of probe channel between two of systems enables concurrent actions of trapping and OCT imaging for the sample. OCT imaging of the sample in depth can achieve by positioning the coherence gating with displacement of reference arm in the OCT interferometer. To demonstrate the efficacy of the system, micron-sized dielectric particles and living cells in solution are simultaneously trapped and optically sliced with cellular resolution.

Citation Download Citation

Woo June Choi, Kwan Seob Park, Tae Joong Eom, Myung-Kyu Oh, and Byeong-Ha Lee "Full-field OCT combined with optical tweezer", Proc. SPIE 8227, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIX, 82271J (2 February 2012); https://doi.org/10.1117/12.908380

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