Single mode and sub-cellular fiber probes for cell scattering and density variation measurements

D. Kokkinos; T. Holden; S. Dehipawala; L. Mora; W. Huazhco; N. Gadura; U. Golebiewska; P. Schneider; G. Tremberger Jr.; D. Lieberman; T. Cheung

doi:10.1117/12.909911

2 February 2012 Single mode and sub-cellular fiber probes for cell scattering and density variation measurements

D. Kokkinos, T. Holden, S. Dehipawala, L. Mora, W. Huazhco, N. Gadura, U. Golebiewska, P. Schneider, G. Tremberger Jr., D. Lieberman, T. Cheung

Author Affiliations +

Proceedings Volume 8230, Biomedical Applications of Light Scattering VI; 82301G (2012) https://doi.org/10.1117/12.909911
Event: SPIE BiOS, 2012, San Francisco, California, United States

Abstract

Cell density is an important parameter in the question of bio-variation and the studying of cell scattering could be a viable tool. The development of spatially resolved optical fiber probe would enable the characterization of optical scattering from cells within a colony. Single mode fiber probe would be budget friendly as compared to a 50-nm sub-cellular fiber probe. This project develops a calibration procedure to correlate the optical scattering measured by a single mode fiber probe to that of a 50-nm sub-cellular fiber probe in the context of cell density variation. The Fourier transform of intensity angular transmission would give correlation information in the Efield in the spatial coordinate. Monte Carlo simulation could be used to constrain the input intensity function spatial content resembling microscopy. The use of a 50-nm sub-cellular fiber probe for detailed study of biological samples would give sub-micron scale length information.

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D. Kokkinos, T. Holden, S. Dehipawala, L. Mora, W. Huazhco, N. Gadura, U. Golebiewska, P. Schneider, G. Tremberger Jr., D. Lieberman, and T. Cheung "Single mode and sub-cellular fiber probes for cell scattering and density variation measurements", Proc. SPIE 8230, Biomedical Applications of Light Scattering VI, 82301G (2 February 2012); https://doi.org/10.1117/12.909911

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