Lab-on-a-chip platforms from sample preparation via continuous-flow PCR to an ultrafast detection of B-agents

Richard Klemm; Holger Becker; Nadine Hlawatsch; Claudia Gärtner

doi:10.1117/12.920790

16 May 2012 Lab-on-a-chip platforms from sample preparation via continuous-flow PCR to an ultrafast detection of B-agents

Richard Klemm, Holger Becker, Nadine Hlawatsch, Claudia Gärtner

Author Affiliations +

Proceedings Volume 8367, Smart Biomedical and Physiological Sensor Technology IX; 83670D (2012) https://doi.org/10.1117/12.920790
Event: SPIE Defense, Security, and Sensing, 2012, Baltimore, Maryland, United States

Abstract

The translation of bio-analytical processes into an automatically functioning microfluidic platform is an attractive task for several reasons. However, due to the complexity of the resulting integrated device covering various process steps like lysis, DNA extraction and purification, continuous-flow PCR and detection etc., these single functional units have to be carefully developed and evaluated in a first step, thus allowing a functional verification prior to final device integration. All the modules as well as the final integrated device have to be manufactured using scalable industrial manufacturing methods, namely injection molding, in order to facilitate commercialization The final integrated device should have a footprint such as SBS-titerplate format, which is generally excepted by the user. For optimal space utilization, microfluidic structures should be on both the top and the bottom side of the device connected with through-holes. The device described in this report is a pathogen DNA analysis example realising all the above prerequisites. Sample is introduced through a Luer-connector in one corner. DNA is extracted in a chamber, which is filled with magnetic beads. All necessary liquid reagents are stored in 500μl modules which are clipped onto the chip. The sample is aliquoted into 8 tracks, liquefies the PCR-reagents which are stored in lyophilized form in small chambers and runs through a meandering channel, in which continuous-flow PCR takes place. The samples are then transferred to the top of the chip and run back to the detection zone where a fluorescence detection of the PCR-products takes place before flowing into waste. As in the device an 8-plex detection is targeted, the spacing of the microchannels after qPCR had to be very narrow in order to have all channels within the field of vision of the detection system.

Citation Download Citation

Richard Klemm, Holger Becker, Nadine Hlawatsch, and Claudia Gärtner "Lab-on-a-chip platforms from sample preparation via continuous-flow PCR to an ultrafast detection of B-agents", Proc. SPIE 8367, Smart Biomedical and Physiological Sensor Technology IX, 83670D (16 May 2012); https://doi.org/10.1117/12.920790

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