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The aim of this study was to examine cellular and matriceal dynamics within pulp tissue of the teeth with repeated
bleaching.
Material and method - The study was made on 25 patients aged between 15 and 45, to whom bleaching method of the
premolars with indication of extraction in orthodontic purposes was applied. None of the subjects smoked and
throughout the investigation no antibiotics had been used. We initiated an intensive oral hygiene program, and we
removed the supragingival and subgingival deposits. Oral hygiene and the gingival health were evaluated before every
session of bleaching. During each visit the dentition was cleaned professionally and if needed the subjects were
reinstucted in proper oral hygiene. After 3 and 5 successive bleachings of the teeth, we removed the dental pulps and we
extracted the premolars. The pulpal biopsies were fixed in buffed formaldehyde 10% for 48 hours, then paraffinized,
sectioned at 3-5 μ and stained with topographic, H&E and trichrome stained. For the electonomicroscopic study we used
the Lehner technique to process the biopsies (n=3) after the reinclusion of the pieces from the paraffine blocks in Epon,
postfixated in buffered glutaraldehyde, micro sectioned at 0,5 μ, contrastated with Pb citrate (stained) and examination in
transmission electronic microscopy with Philips microscope.
Results - At cellular and matriceal level we observed a marked collagen fibrillogenesis in the presence of active
fibroblasts, with well developed cellular organites and fibroclastic aspects which suggest matriceal active repair.
The microvascular network presents an activated endothelium with turgescent endothelial cells, with intracitoplasmatic
resorbtion vacuols, well developed Golgi Complex.
Conclusion - We interpreed the cell - matriceal lesions in the context of the acute inflammatory process in the first
lesional phase and chronic scleroatrophic process after successive bleaching.
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