Plasma surface reflectance spectroscopy for non-invasive and continuous monitoring of extracellular component of blood

Daisuke Sakota; Setsuo Takatani

doi:10.1117/12.921331

9 May 2012 Plasma surface reflectance spectroscopy for non-invasive and continuous monitoring of extracellular component of blood

Daisuke Sakota, Setsuo Takatani

Author Affiliations +

Proceedings Volume 8439, Optical Sensing and Detection II; 84392K (2012) https://doi.org/10.1117/12.921331
Event: SPIE Photonics Europe, 2012, Brussels, Belgium

Abstract

To achieve the quantitative optical non-invasive diagnosis of blood during extracorporeal circulation therapies, the instrumental technique to extract extracellular spectra from whole blood was developed. In the circuit, the continuous blood flow was generated by a centrifugal blood pump. The oxygen saturation was maintained 100% by an oxygenator. The developed glass optical flow cell was attached to the outlet tubing of the oxygenator. The halogen lamp including the light from 400 to 900 nm wavelength was used for the light source. The light was guided into an optical fiber. The light emitted by the fiber was collimated and emitted to the flow cell flat surface at the incident angle of 45 degrees. The light just reflected on the boundary between inner surface of the flow cell and plasma at 45 degrees was detected by the detection fiber. The detected light was analyzed by a spectral photometer. The obtained spectrum from 400 to 600nm wavelength was not changed with respect to the hematocrit. In contrast, the signal in the spectral range was changed when the plasma free hemoglobin increased. By using two spectral range, 505±5 nm and 542.5±2.5 nm, the differential spectrum was correlated with the free hemoglobin at R²=0.99. On the other hand, as for the hematocrit, the differential spectrum was not correlated at R²=0.01. Finally, the plasma free hemoglobin was quantified with the accuracy of 22±19mg/dL. The result shows that the developed plasma surface reflectance spectroscopy (PSRS) can extract the plasma spectrum from flowing whole blood.

Citation Download Citation

Daisuke Sakota and Setsuo Takatani "Plasma surface reflectance spectroscopy for non-invasive and continuous monitoring of extracellular component of blood", Proc. SPIE 8439, Optical Sensing and Detection II, 84392K (9 May 2012); https://doi.org/10.1117/12.921331

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available