Motion compensation of optical mapping signals from rat heart slices

B. Stender; M. Brandenburger; B. Wang; Z. Zhang; A. Schlaefer

doi:10.1117/12.2008992

11 December 2012 Motion compensation of optical mapping signals from rat heart slices

B. Stender, M. Brandenburger, B. Wang, Z. Zhang, A. Schlaefer

Proceedings Volume 8553, Optics in Health Care and Biomedical Optics V; 855305 (2012) https://doi.org/10.1117/12.2008992
Event: Photonics Asia, 2012, Beijing, China

Abstract

Optical mapping is a well established technique for recording monophasic action potential traces either within myocardial slices or on the epicardial surface of isolated hearts. This measuring technique offers a high spatial and sufficient temporal resolution but it is sensitive towards myocardial motion. Motion artifacts occur because the mapping between a certain tissue portion sending out fluorescent light and a pixel of the photodetector changes over time. So far this problem has been adressed in two different ways: Suppressing the motion or ratiometric imaging. Working with beating rat heart slices we developed a different approach to noninvasively record simultaneously optical mapping data and motion of the slices. Our approach is based on image registration. We use the background fluorescent light to perform first a rigid transformation to detect translational and and rotational motion. The contractive motion is recovered using a non-rigid demons registration algorithm afterwards. The investigation is based on recordings of four different slices stained with Di-4-ANEPPS.

Citation Download Citation

B. Stender, M. Brandenburger, B. Wang, Z. Zhang, and A. Schlaefer "Motion compensation of optical mapping signals from rat heart slices", Proc. SPIE 8553, Optics in Health Care and Biomedical Optics V, 855305 (11 December 2012); https://doi.org/10.1117/12.2008992

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
6 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Heart

Tissue optics

Image registration

Image segmentation

Modulation transfer functions

Tissues

Action potentials

Show All Keywords

Keywords/Phrases

Search In:

Publication Years