Curvature phase factor in digital holographic microscopy

Ana Doblas; Emilio Sánchez-Ortiga; Manuel Martínez-Corral; Genaro Saavedra; Pedro Andres; Jorge Garcia-Sucerquia

doi:10.1117/12.2026176

18 November 2013 Curvature phase factor in digital holographic microscopy

Ana Doblas, Emilio Sánchez-Ortiga, Manuel Martínez-Corral, Genaro Saavedra, Pedro Andres, Jorge Garcia-Sucerquia

Proceedings Volume 8785, 8th Iberoamerican Optics Meeting and 11th Latin American Meeting on Optics, Lasers, and Applications; 87856Q (2013) https://doi.org/10.1117/12.2026176
Event: 8th Ibero American Optics Meeting/11th Latin American Meeting on Optics, Lasers, and Applications, 2013, Porto, Portugal

Abstract

As digital holographic microscopy (DHM) uses microscope objectives (MO) for enlarging the sample, some associated effects that are not present in optical microscopy have to be considered as quantitative phase imaging (QPI) is regarded. The remaining phase curvature introduced by the MO, which does not affect the optical microscopes, plays a determinant role in the performance of the DHM. In this contribution a thorough analysis of the physical parameters that control the appropriate utilization of MOs in DHM is presented. The analysis is carried for QPI. We study the sample phase perturbations that the MO phase curvature introduces. An analysis of the regular ways as these phase anomalies are removed is presented. The study is supported by means of numerical modeling and experimental results.

Citation Download Citation

Ana Doblas, Emilio Sánchez-Ortiga, Manuel Martínez-Corral, Genaro Saavedra, Pedro Andres, and Jorge Garcia-Sucerquia "Curvature phase factor in digital holographic microscopy", Proc. SPIE 8785, 8th Iberoamerican Optics Meeting and 11th Latin American Meeting on Optics, Lasers, and Applications, 87856Q (18 November 2013); https://doi.org/10.1117/12.2026176

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