Mouse neuroblastoma cell based model and the effect of epileptic events on calcium oscillations and neural spikes

Suhwan Kim; Juyeong Baek; Unsang Jung; Sangwon Lee; Woonggyu Jung; Jeehyun Kim; Shinwon Kang

doi:10.1117/12.2017666

20 May 2013 Mouse neuroblastoma cell based model and the effect of epileptic events on calcium oscillations and neural spikes

Suhwan Kim, Juyeong Baek, Unsang Jung, Sangwon Lee, Woonggyu Jung, Jeehyun Kim, Shinwon Kang

Author Affiliations +

Proceedings Volume 8879, Nano-Bio Sensing, Imaging, and Spectroscopy; 88790T (2013) https://doi.org/10.1117/12.2017666
Event: Nano-Bio Sensing, Imaging and Spectroscopy, 2013, Jeju, Korea, Republic of

Abstract

Recently, Mouse neuroblastoma cells are considered as an attractive model for the study of human neurological and prion diseases, and intensively used as a model system in different areas. Among those areas, differentiation of neuro2a (N2A) cells, receptor mediated ion current, and glutamate induced physiological response are actively investigated. The reason for the interest to mouse neuroblastoma N2A cells is that they have a fast growing rate than other cells in neural origin with a few another advantages. This study evaluated the calcium oscillations and neural spikes recording of mouse neuroblastoma N2A cells in an epileptic condition. Based on our observation of neural spikes in mouse N2A cell with our proposed imaging modality, we report that mouse neuroblastoma N2A cells can be an important model related to epileptic activity studies. It is concluded that the mouse neuroblastoma N2A cells produce the epileptic spikes in vitro in the same way as produced by the neurons or the astrocytes. This evidence advocates the increased and strong level of neurotransmitters release by enhancement in free calcium using the 4-aminopyridine which causes the mouse neuroblastoma N2A cells to produce the epileptic spikes and calcium oscillation.

Citation Download Citation

Suhwan Kim, Juyeong Baek, Unsang Jung, Sangwon Lee, Woonggyu Jung, Jeehyun Kim, and Shinwon Kang "Mouse neuroblastoma cell based model and the effect of epileptic events on calcium oscillations and neural spikes", Proc. SPIE 8879, Nano-Bio Sensing, Imaging, and Spectroscopy, 88790T (20 May 2013); https://doi.org/10.1117/12.2017666

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
8 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Calcium

Epilepsy

Neurons

Proteins

Confocal microscopy

Ions

Microscopes

Show All Keywords

Keywords/Phrases

Search In:

Publication Years