Label-free molecular imaging

Junqi Zhang; Qi Li; Rongxin Fu; Tongzhou Wang; Ruliang Wang; Guoliang Huang

doi:10.1117/12.2038462

12 March 2014 Label-free molecular imaging

Junqi Zhang, Qi Li, Rongxin Fu, Tongzhou Wang, Ruliang Wang, Guoliang Huang

Proceedings Volume 8949, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXI; 894917 (2014) https://doi.org/10.1117/12.2038462
Event: SPIE BiOS, 2014, San Francisco, California, United States

Abstract

Optical microscopy technology has achieved great improvements in the 20th century. The detection limit has reached about twenty nanometers (with near-field optics, STED, PALM and STORM). But in the application areas such as life science, medical science, clinical treatment and especially in vivo dynamic measurement, mutual restrictions still exist between numeric aperture/magnification and working distance, fluorescent dependent, and between resolution and frame rate/field size, etc. This paper explores a hyperspectral scanning super-resolution label free molecules imaging method based on the white light interferometry. The vertical detection resolution was approximate to 1 nm which is the thickness of a single molecular layer and dynamic measuring range of thickness reaches to 10 μm. The spectrum-shifting algorithm is developed for robust restructure of images when the pixels are overlapped. Micro-biochip with protein binding and DNA amplification could be detected by using this spectral scanning super-resolution molecules imaging in label free. This method has several advantages as following: Firstly, the decoding and detecting steps are combined into one step. It makes tests faster and easier. Secondly, we used thickness-coded, minimized chips instead of a large microarray chip to carry the probes. This accelerates the interaction of the biomolecules. Thirdly, since only one kind of probes are attached to our thickness-coded, minimized chip, users can only pick out the probes they are interested in for a test without wasting unnecessary probes and chips.

Citation Download Citation

Junqi Zhang, Qi Li, Rongxin Fu, Tongzhou Wang, Ruliang Wang, and Guoliang Huang "Label-free molecular imaging", Proc. SPIE 8949, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXI, 894917 (12 March 2014); https://doi.org/10.1117/12.2038462

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