Lab-on-a-chip modules for detection of highly pathogenic bacteria: from sample preparation to detection

S. Julich; R. Kopinč; N. Hlawatsch; C. Moche; A. Lapanje; C. Gärtner; H. Tomaso

doi:10.1117/12.2049859

22 May 2014 Lab-on-a-chip modules for detection of highly pathogenic bacteria: from sample preparation to detection

S. Julich, R. Kopinč, N. Hlawatsch, C. Moche, A. Lapanje, C. Gärtner, H. Tomaso

Proceedings Volume 9107, Smart Biomedical and Physiological Sensor Technology XI; 91070R (2014) https://doi.org/10.1117/12.2049859
Event: SPIE Sensing Technology + Applications, 2014, Baltimore, MD, United States

Abstract

Lab-on-a-chip systems are innovative tools for the detection and identification of microbial pathogens in human and veterinary medicine. The major advantages are small sample volume and a compact design. Several fluidic modules have been developed to transform analytical procedures into miniaturized scale including sampling, sample preparation, target enrichment, and detection procedures. We present evaluation data for single modules that will be integrated in a chip system for the detection of pathogens. A microfluidic chip for purification of nucleic acids was established for cell lysis using magnetic beads. This assay was evaluated with spiked environmental aerosol and swab samples. Bacillus thuringiensis was used as simulant for Bacillus anthracis, which is closely related but non-pathogenic for humans. Stationary PCR and a flow-through PCR chip module were investigated for specific detection of six highly pathogenic bacteria. The conventional PCR assays could be transferred into miniaturized scale using the same temperature/time profile. We could demonstrate that the microfluidic chip modules are suitable for the respective purposes and are promising tools for the detection of bacterial pathogens. Future developments will focus on the integration of these separate modules to an entire lab-on-a-chip system.

Citation Download Citation

S. Julich, R. Kopinč, N. Hlawatsch, C. Moche, A. Lapanje, C. Gärtner, and H. Tomaso "Lab-on-a-chip modules for detection of highly pathogenic bacteria: from sample preparation to detection", Proc. SPIE 9107, Smart Biomedical and Physiological Sensor Technology XI, 91070R (22 May 2014); https://doi.org/10.1117/12.2049859

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available