Ex vivo optical characterization of in vivo grown tissues on dummy sensor implants using double integrating spheres measurement

Sandeep Sharma; Mohammad Goodarzi; Ben Aernouts; Karolien Gellynck; Lieven Vlaminck; Ronny Bockstaele; Maria Cornelissen; Herman Ramon; Wouter Saeys

doi:10.1117/12.2052239

8 May 2014 Ex vivo optical characterization of in vivo grown tissues on dummy sensor implants using double integrating spheres measurement

Sandeep Sharma, Mohammad Goodarzi, Ben Aernouts, Karolien Gellynck, Lieven Vlaminck, Ronny Bockstaele, Maria Cornelissen, Herman Ramon, Wouter Saeys

Author Affiliations +

Proceedings Volume 9129, Biophotonics: Photonic Solutions for Better Health Care IV; 91292A (2014) https://doi.org/10.1117/12.2052239
Event: SPIE Photonics Europe, 2014, Brussels, Belgium

Abstract

Near infrared spectroscopy offers a promising technological platform for continuous glucose monitoring in the human body. NIR measurements can be performed in vivo with an implantable single-chip based optical NIR sensor. However, the application of NIR spectroscopy for accurate estimation of the analyte concentration in highly scattering biological systems still remains a challenge. For instance, a thin tissue layer may grow in the optical path of the sensor. As most biological tissues allow only a small fraction of the collimated light to pass, this might result in a large reduction of the light throughput. To quantify the effect of presence of a thin tissue layer in the optical path, the bulk optical properties of tissue samples grown on sensor dummies which had been implanted for several months in goats were characterized using Double Integrating Spheres and unscattered transmittance measurements. The measured values of diffuse reflectance, diffuse transmittance and collimated transmittance were used as input to Inverse Adding-Doubling algorithm to estimate the bulk optical properties of the samples. The estimates of absorption and scattering coefficients were then used to calculate the light attenuation through a thin tissue layer. Based on the lower reduction in unscattered transmittance and higher absorptivity of glucose molecules, the measurement in the combination band was found to be the better option for the implantable sensor. As the tissues were found to be highly forward scattering with very low unscattered transmittance, the diffuse transmittance measurement based sensor configuration was recommended for the implantable glucose sensor.

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Sandeep Sharma, Mohammad Goodarzi, Ben Aernouts, Karolien Gellynck, Lieven Vlaminck, Ronny Bockstaele, Maria Cornelissen, Herman Ramon, and Wouter Saeys "Ex vivo optical characterization of in vivo grown tissues on dummy sensor implants using double integrating spheres measurement", Proc. SPIE 9129, Biophotonics: Photonic Solutions for Better Health Care IV, 91292A (8 May 2014); https://doi.org/10.1117/12.2052239

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