Nanoparticle-assisted-multiphoton microscopy for in vivo brain imaging of mice

Jun Qian

doi:10.1117/12.2181331

10 March 2015 Nanoparticle-assisted-multiphoton microscopy for in vivo brain imaging of mice

Jun Qian

Proceedings Volume 9305, Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics II; 93051J (2015) https://doi.org/10.1117/12.2181331
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

Neuro/brain study has attracted much attention during past few years, and many optical methods have been utilized in order to obtain accurate and complete neural information inside the brain. Relying on simultaneous absorption of two or more near-infrared photons by a fluorophore, multiphoton microscopy can achieve deep tissue penetration and efficient light detection noninvasively, which makes it very suitable for thick-tissue and in vivo bioimaging. Nanoparticles possess many unique optical and chemical properties, such as anti-photobleaching, large multiphoton absorption cross-section, and high stability in biological environment, which facilitates their applications in long-term multiphoton microscopy as contrast agents. In this paper, we will introduce several typical nanoparticles (e.g. organic dye doped polymer nanoparticles and gold nanorods) with high multiphoton fluorescence efficiency. We further applied them in two- and three-photon in vivo functional brain imaging of mice, such as brain-microglia imaging, 3D architecture reconstruction of brain blood vessel, and blood velocity measurement.

Citation Download Citation

Jun Qian "Nanoparticle-assisted-multiphoton microscopy for in vivo brain imaging of mice", Proc. SPIE 9305, Optical Techniques in Neurosurgery, Neurophotonics, and Optogenetics II, 93051J (10 March 2015); https://doi.org/10.1117/12.2181331

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KEYWORDS

In vivo imaging

Brain

Nanoparticles

Neuroimaging

Microscopy

Multiphoton microscopy

Absorption

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