Fluorescence lifetime imaging of deep-seated fluorophore in turbid medium

Alexander V. Khilov; I. I. Fiks; Vladimir I. Plehanov; M. Yu. Kirillin; I. V. Turchin

doi:10.1117/12.2075522

5 March 2015 Fluorescence lifetime imaging of deep-seated fluorophore in turbid medium

Alexander V. Khilov, I. I. Fiks, Vladimir I. Plehanov, M. Yu. Kirillin, I. V. Turchin

Proceedings Volume 9319, Optical Tomography and Spectroscopy of Tissue XI; 93190S (2015) https://doi.org/10.1117/12.2075522
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

Correct identification of different fluorophores in the fluorescence lifetime imaging in vivo requires accounting for distortion of the measured fluorescent kinetics curve due to light scattering and absorption in medium. This distortion induces the difference between real and measured lifetimes of a fluorophore. We obtained analytical expression based on diffuse approximation of radiation transfer equation that allows to refine estimating the lifetime of a fluorophore. It was shown that our approach can be applied both for analytic kinetics curves obtained by diffuse approximation, Monte Carlo simulated curves and results of model experiment. Analytical and Monte Carlo simulated curves were obtained for media with different optical properties and lifetimes corresponding to those of real fluorophores. Results of numerical simulation are confirmed by the results of the model experiment.

Citation Download Citation

Alexander V. Khilov, I. I. Fiks, Vladimir I. Plehanov, M. Yu. Kirillin, and I. V. Turchin "Fluorescence lifetime imaging of deep-seated fluorophore in turbid medium", Proc. SPIE 9319, Optical Tomography and Spectroscopy of Tissue XI, 93190S (5 March 2015); https://doi.org/10.1117/12.2075522

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KEYWORDS

Luminescence

Monte Carlo methods

Picosecond phenomena

Absorption

Distortion

Fluorescence lifetime imaging

Optical properties

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