Molecular constituents of colorectal cancer metastatic to the liver by imaging infrared spectroscopy

James V. Coe; Zhaomin Chen; Ran Li; Steven V Nystrom; Ryan Butke; Barrie Miller; Charles L. Hitchcock; Heather C. Allen; Stephen P. Povoski; Edward W. Martin Jr.

doi:10.1117/12.2079884

2 March 2015 Molecular constituents of colorectal cancer metastatic to the liver by imaging infrared spectroscopy

James V. Coe, Zhaomin Chen, Ran Li, Steven V Nystrom, Ryan Butke, Barrie Miller, Charles L. Hitchcock, Heather C. Allen, Stephen P. Povoski, Edward W. Martin Jr.

Author Affiliations +

Proceedings Volume 9328, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIII; 93280R (2015) https://doi.org/10.1117/12.2079884
Event: SPIE BiOS, 2015, San Francisco, California, United States

Abstract

Infrared (IR) imaging spectroscopy of human liver tissue slices has been used to identify and characterize liver metastasis of colorectal origin which was surgically removed from a consenting patient and frozen without formalin fixation or dehydration procedures, so that lipids and water remain in the tissues. First, a k-means clustering analysis, using metrics from the IR spectra, identified groups within the image. The groups were identified as tumor or nontumor regions by comparing to an H and E stain of the same sample after IR imaging. Then, calibrant IR spectra of protein, several fats, glycogen, and polyvinyl alcohol were isolated by differencing spectra from different regions or groups in the image space. Finally, inner products (or scores) of the IR spectra at each pixel in the image with each of the various calibrants were calculated showing how the calibrant molecules vary in tumor and nontumor regions. In this particular case, glycogen and protein changes enable separation of tumor and nontumor regions as shown with a contour plot of the glycogen scores versus the protein scores.

Citation Download Citation

James V. Coe, Zhaomin Chen, Ran Li, Steven V Nystrom, Ryan Butke, Barrie Miller, Charles L. Hitchcock, Heather C. Allen, Stephen P. Povoski, and Edward W. Martin Jr. "Molecular constituents of colorectal cancer metastatic to the liver by imaging infrared spectroscopy", Proc. SPIE 9328, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XIII, 93280R (2 March 2015); https://doi.org/10.1117/12.2079884

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